A simple and quick isolation method to obtain a huge number of fibroblasts like mesenchymal stem cells from human umbilical cord

In this publication, we have developed a simple and quick isolation method of stem cells from the umbilical cord. Usually, cord is waste product during child delivery procedure, but cords contain valuable called mesenchymal stroma (also MSCs), which can be basis for different applications e.g., testing effect therapeutic compounds as well development cure degenerative diseases like bone cartilage related. These used to create new artificial organs too. The was cut with sterile scissors into small pieces (1-2 mm size) these were placed on 6 culture plates. cell media added plates after 5-10 minutes placing in CO2 incubator. successful growth observed 7 days wells, where implanted. Microscopic observations show how are oozing out pieces. isolated passaged further. adhere plastic surface, fibroblast shaped, successfully experiments.